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Updated: Apr 20, 2026
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
Published on: July 17, 2021
Performance testing of a semi-automatic card punch system, using direct STR profiling of DNA from blood samples on
Samantha J Ogden1, Jeffrey K Horton, Simon L Stubbs
1GE Healthcare Life Sciences R&D, The Maynard Centre, Forest Farm, Whitchurch, Cardiff, CF14 7YT, U.K.
Abstract:
The 1.2 mm Electric Coring Tool (e-Core™) was developed to increase the throughput of FTA(™) sample collection cards used during forensic workflows and is similar to a 1.2 mm Harris manual micro-punch for sampling dried blood spots. Direct short tandem repeat (STR) DNA profiling was used to compare samples taken by the e-Core tool with those taken by the manual micro-punch. The performance of the e-Core device was evaluated using a commercially available PowerPlex™ 18D STR System. In addition, an analysis was performed that investigated the potential carryover of DNA via the e-Core punch from one FTA disc to another. This contamination study was carried out using Applied Biosystems AmpflSTR™ Identifiler™ Direct PCR Amplification kits. The e-Core instrument does not contaminate FTA discs when a cleaning punch is used following excision of discs containing samples and generates STR profiles that are comparable to those generated by the manual micro-punch.
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