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Related Experiment Video

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The soft agar colony formation assay.

Stanley Borowicz1, Michelle Van Scoyk2, Sreedevi Avasarala2

  • 1Department of Hematology and Oncology, University of Illinois at Chicago.

Journal of Visualized Experiments : Jove
|November 20, 2014
PubMed
Summary
This summary is machine-generated.

Wnt7A and Frizzled-9 (Fzd-9) suppress tumor growth. Overexpressing these Wnt pathway components inhibited anchorage-independent growth in a murine lung carcinoma cell line, demonstrating their tumor suppressive effects.

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Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Biology

Background:

  • Anchorage-independent growth is a key hallmark of cancer.
  • The soft agar colony formation assay is a standard in vitro method to assess malignant transformation.
  • This assay allows for semi-quantitative evaluation of cellular growth under various conditions.

Purpose of the Study:

  • To demonstrate the tumor suppressive effects of Wnt7A and Frizzled-9 (Fzd-9) in a murine lung carcinoma model.
  • To utilize the soft agar colony formation assay to evaluate these effects.

Main Methods:

  • Soft agar colony formation assay was performed using the CMT167 murine lung carcinoma cell line.
  • Concurrent overexpression of Wnt7a and Frizzled-9 (Fzd-9) was induced in the cells.

Main Results:

  • Concurrent overexpression of Wnt7a and Fzd-9 significantly inhibited colony formation in CMT167 cells.
  • This inhibition indicates a suppression of anchorage-independent growth.

Conclusions:

  • Wnt7a ligand and its Frizzled-9 (Fzd-9) receptor are sufficient to suppress tumor growth in this murine lung carcinoma model.
  • The Wnt signaling pathway, specifically Wnt7a and Fzd-9, plays a role in tumor suppression.