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Epidermal growth factor activates calcium-permeable channels in A 431 cells
G N Mozhayeva1, A P Naumov, Kuryshev YuA
1Academy of Sciences of the U.S.S.R., Institute of Cytology, Leningrad.
Abstract:
The patch clamp technique in a cell-attached configuration was used to search for calcium-permeable channels in human carcinoma A 431 cells. Unitary inward currents were recorded with 100 mM CaCl2 in a pipette, with the mean slope conductance of 2.8 pS and a reversal potential (obtained by extrapolation) of +25.5 mV. Application of epidermal growth factor (EGF) into the extracellular solution produced a transient increase in the probability of these channels being open. The effect develops with delay of about 20 s and lasts thereafter for 36 s (mean values). We propose that these channels mediate an EGF-induced increase in the concentration of cytosolic free calcium.
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