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Updated: Apr 20, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
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Development of new natural extracts.

Sophie Lavoine-Hanneguelle1, Christine Périchet, Nicolas Schnaebele

  • 1R&D Department, Charabot S.A., 10 avenue Yves Emmanuel Baudoin, FR-06130, Grasse.

Chemistry & Biodiversity
|November 20, 2014
PubMed
Summary

New eco-friendly extraction methods, like supercritical CO2, enable the use of fresh plants for natural flavor and fragrance ingredients. This innovation addresses market trends and regulatory demands for sustainable sourcing.

Keywords:
BiosolventsJasmineOrange blossomSambacSupercritical CO2

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Area of Science:

  • Natural Products Chemistry
  • Green Extraction Technologies
  • Flavor and Fragrance Industry

Background:

  • The flavor and fragrance industry traditionally relied on synthetic molecules for innovation.
  • Natural products were often seen as static, protected by proprietary knowledge.
  • Market trends towards ecology and new regulations are driving change.

Purpose of the Study:

  • To develop novel, eco-friendly extraction processes for natural products.
  • To adapt to regulatory constraints and sustainability demands (e.g., hexane-free).
  • To explore the potential of fresh plant materials for high-value extracts.

Main Methods:

  • Supercritical CO2 extraction applied to fresh plant materials (Jasmine Grandiflorum, Jasmine Sambac, Orange blossom).
  • Analytical techniques including Gas Chromatography (GC), GC/Mass Spectrometry (GC/MS), GC-Olfactometry (GC-O), and High-Performance Thin-Layer Chromatography (HPTLC).
  • Exploration of new raw materials with subsequent safety assessments.

Main Results:

  • Successful development of industrial-scale, eco-friendly flower extracts using fresh plants.
  • Characterization of extracts using advanced analytical methods.
  • Demonstration of feasibility for using novel raw materials, pending safety evaluations.

Conclusions:

  • Supercritical CO2 extraction offers a viable, sustainable alternative for producing natural flavor and fragrance ingredients.
  • This approach fosters creativity and innovation in the industry by enabling the use of fresh botanicals.
  • The developed methods support the industry's shift towards ecological practices and regulatory compliance.