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How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells
Published on: January 7, 2019
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Quantification of fluorescent reporters in plant cells
Michael Pound1, Andrew P French, Darren M Wells
1Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, UK.
Methods in Molecular Biology (Clifton, N.J.)
|November 20, 2014
Abstract:
Fluorescent reporters are powerful tools for plant research. Many studies require accurate determination of fluorescence intensity and localization. Here, we describe protocols for the quantification of fluorescence intensity in plant cells from confocal laser scanning microscope images using semiautomated software and image analysis techniques.

