Related Experiment Video

Updated: Apr 20, 2026

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution
10:47

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

Published on: May 5, 2023

4.8K

A mitochondrial strategy for safeguarding the reprogrammed genome

Alessandro Prigione1, James Adjaye2

  • 1Max Delbrueck Center for Molecular Medicine (MDC), Robert-Roessle-Str. 10, D-13125 Berlin-Buch, Germany.

Cell Regeneration (London, England)
|November 20, 2014
PubMed

Insights

Somatic cell reprogramming causes genomic issues, hindering regenerative medicine. A study in Stem Cell Reports proposes using antioxidants to balance mitochondrial and oxidative stress, counteracting these aberrations.

Area of Science:

  • Biomedical research
  • Stem cell biology
  • Regenerative medicine

Background:

  • Somatic cell reprogramming is crucial for regenerative medicine but can induce genomic aberrations.
  • These aberrations pose a significant challenge to the safe and effective clinical application of reprogrammed cells.

Purpose of the Study:

  • To investigate a strategy for counteracting genomic aberrations during somatic cell reprogramming.
  • To explore the role of the mitochondrial/oxidative stress pathway in reprogramming-induced genomic instability.

Main Methods:

  • The study by Ji et al. focused on analyzing genomic integrity following somatic cell reprogramming.
  • They investigated the effects of modulating the mitochondrial/oxidative stress pathway.
  • Antioxidant supplementation was employed as a key experimental intervention.

Main Results:

  • Genomic aberrations were observed as a consequence of somatic cell reprogramming.
  • Balancing the mitochondrial/oxidative stress pathway demonstrated a counteracting effect on these aberrations.
  • Antioxidant supplementation proved effective in mitigating reprogramming-induced genomic instability.

Conclusions:

  • Modulating the mitochondrial/oxidative stress pathway is a viable strategy to address genomic aberrations in somatic cell reprogramming.
  • Antioxidant supplementation offers a promising approach to enhance the safety and applicability of regenerative medicine technologies.
Keywords:
AntioxidantsGenomic aberrationsMitochondriaReprogrammingiPSCs

More Related Videos

An In Vitro Approach to Study Mitochondrial Dysfunction: A Cybrid Model06:05

An In Vitro Approach to Study Mitochondrial Dysfunction: A Cybrid Model

Published on: March 9, 2022

4.6K
Author Spotlight: Transmitochondrial Cybrid Generation Using Cancer Cell Lines07:49

Author Spotlight: Transmitochondrial Cybrid Generation Using Cancer Cell Lines

Published on: March 17, 2023

3.4K

Related Concept Videos

Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
10.2K
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
19.5K
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
208.8K
Export of Mitochondrial and Chloroplast Genes02:19

Export of Mitochondrial and Chloroplast Genes

A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
4.5K
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
4.7K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.3K