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Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
Published on: March 10, 2020
[Acute gastroenteritis by Cambylobacter spp: a retrospective study of a paediatric emergency department]
Ana Teresa Soares1, Catarina Couto1, Patrícia Romão2
1Serviço de Pediatria. Hospital Garcia de Orta. Almada. Portugal.
Insights
Campylobacter is a leading cause of bacterial gastroenteritis in children. While often self-resolving, severe cases and rising quinolone resistance warrant attention.
Area of Science:
- Pediatric Infectious Diseases
- Microbiology
- Public Health
Background:
- Campylobacter infection is a global health concern.
- It is the primary cause of acute bacterial gastroenteritis in the European Union.
Purpose of the Study:
- To determine Campylobacter prevalence in pediatric gastroenteritis cases.
- To analyze microbiological, epidemiological, clinical, and therapeutic profiles.
- To identify associated complications.
Main Methods:
- Retrospective review of clinical files.
- Inclusion criteria: pediatric emergency department patients with acute gastroenteritis and isolated Campylobacter in stool.
- Study period: 30 months.
Main Results:
- Campylobacter was identified in 49% of 216 stool cultures.
- Bloody diarrhea (90%) and fever (47%) were common symptoms.
- One patient developed sepsis; 8 received azithromycin.
Conclusions:
- Campylobacter is the predominant bacterial isolate in pediatric gastroenteritis.
- Most infections are self-limiting, but severe forms occur.
- Increasing quinolone resistance is a significant concern.
Introduction:
Infection by Cambylobacter occurs worldwide and represents the main cause of acute bacterial gastroenteritis within the European Union.
Aims:
Determine the prevalence of Campylobacter in stool cultures from patients with gastroenteritis and study their microbiological, epidemiological, clinical, and therapeutic profiles, as well as associated complications.
Material And Methods:
Review of clinical files of patients presenting to the paediatric emergency department of a general district hospital over a 30 month period with an acute gastroenteritis and a Campylobacter isolated in a stool specimen.
Results:
Out of 216 stool cultures, 98 (45%) were positive. We identified Campylobacter in 49 (50%) cases; 30 (61%) were female. Median age was 23 months. Fourteen patients were under one year of age, 25 between one and five years old and 10 patients were over five years old. Watery diarrhea was identified in 5 (10%) patients, bloody diarrhea in 44 (90%) and mucosanguineous in 14 (29%), while 23 (47%) had fever, 14 (29%) complained of abdominal pain and 11 (22%) presented with vomiting. One patient was septic. Five patients were admitted as inpatients. Eight patients were treated with azithromycin.
Discussion:
This is the largest published national series on gastroenteritis by Campylobacter in children and the first in the south region. Campylobacter was the main bacteria isolated. Infection was self-resolving in the majority of cases. Nevertheless, severe forms of this infection should be considered. Increased resistance to quinolones is worrisome.
Conclusion:
Judicious use of stool cultures allows etiological identification of bacterial gastroenteritis. The increase in Campylobacter cases reinforces the need for better control of hygiene measures in handling food products.
