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Related Concept Videos

CRISPR and crRNAs02:53

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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Related Experiment Video

Updated: Apr 20, 2026

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira
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Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

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Specific gene repression by CRISPRi system transferred through bacterial conjugation.

Weiyue Ji1, Derrick Lee, Eric Wong

  • 1Center for Systems and Synthetic Biology, ‡Department of Cellular and Molecular Pharmacology, §Department of Biochemistry and Biophysics, ∥Department of Bioengineering and Therapeutic Sciences, ⊥Howard Hughes Medical Institute, University of California, San Francisco , San Francisco, California 94158, United States.

ACS Synthetic Biology
|November 20, 2014
PubMed
Summary

Researchers developed a new CRISPR interference (CRISPRi) tool delivered via bacterial conjugation. This method precisely targets and represses specific genes in Escherichia coli, offering a novel approach for bacterial population control without broad-spectrum antibiotics.

Keywords:
CRISPR/Cas9conjugationhorizontal gene transfersynthetic biologysynthetic gene regulation

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Synthetic Biology

Background:

  • Current bacterial population control relies on broad-spectrum antibiotics, often disrupting beneficial microbes.
  • Targeting specific bacterial strains without collateral damage to the microbiome remains a challenge.

Purpose of the Study:

  • To develop a novel method for precise gene regulation in specific bacterial strains.
  • To establish a tool for targeted bacterial population control using horizontal gene transfer.

Main Methods:

  • Utilized bacterial conjugation, a natural DNA transfer process, to deliver an engineered CRISPR interference (CRISPRi) system.
  • Introduced the CRISPRi system into recipient Escherichia coli cells to target specific genes.

Main Results:

  • Successfully delivered the engineered CRISPRi system into recipient Escherichia coli cells.
  • Demonstrated specific repression of a reporter gene in recipient cells using the CRISPRi system.
  • Established a functional tool for gene regulation across bacterial populations.

Conclusions:

  • Bacterial conjugation can effectively deliver engineered CRISPRi systems for targeted gene repression.
  • This approach offers a promising new strategy for controlling specific bacterial populations and managing microbiomes.
  • The developed tool has potential applications in synthetic biology and microbiome engineering.