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Related Experiment Video

Updated: Apr 20, 2026

Assembly and Characterization of Polyelectrolyte Complex Micelles

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

Recent developments in nucleic acid delivery with polyethylenimines.

Patrick Neuberg¹, Antoine Kichler¹

¹Laboratoire "Vecteurs: Synthèse et Applications Thérapeutiques", UMR7199 CNRS-Université de Strasbourg, Faculté de Pharmacie, Illkirch, France.

Advances in Genetics

|November 21, 2014

Summary

This summary is machine-generated.

Polyethylenimines (PEIs) are effective nucleic acid delivery agents with a good safety profile in clinical trials. Their "proton sponge" mechanism enhances endosomal escape, making them versatile for gene therapy applications.

Keywords:

Antisense oligonucleotides Cationic polymers Endosomal release Gene therapy Nonviral gene delivery Polyethylenimines Proton sponge effect RNA interference and siRNA

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Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

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Published on: March 1, 2013

Area of Science:

Biotechnology
Polymer Chemistry
Gene Therapy

Background:

Polyethylenimines (PEIs) are widely recognized for their efficiency in delivering nucleic acids.
Despite concerns regarding biodegradability, PEIs have demonstrated a favorable safety profile in clinical settings.

Purpose of the Study:

To elucidate the mechanism of PEI-mediated nucleic acid transfection.
To review recent advancements in the application of PEIs for delivering DNA, antisense oligonucleotides, and siRNA.

Main Methods:

Discussion of the "proton sponge" effect, a key mechanism for endosomal escape.
Overview of chemical modifications and conjugations possible with PEIs due to their amino groups.

Main Results:

PEIs exhibit high transfection efficiency attributed to their buffering capacity within endosomes.
The "proton sponge" effect facilitates endosomal escape of nucleic acid/PEI complexes (polyplexes).

Conclusions:

PEIs are versatile and efficient agents for nucleic acid delivery, with potential in gene therapy.
Ongoing research focuses on optimizing PEI-based delivery systems for therapeutic applications.