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A human embryo fibroblast-derived peptide suppresses the zymosan-induced biochemical activation in human
1Toxicological Research Laboratories, Kyowa Hakko Kogyo Co., Yamaguchi, Japan.
Abstract:
Previously, a low-molecular-weight peptide was found in the serum-free culture media conditioned by a human embryo fibroblast cell strain (YH-1), which showed inhibitory effects on the proliferation and functions of B and T lymphocytes (Okai et al. (1987) Zool. Sci. 4, 99-105). In this paper, the inhibitory effect of this peptide on the functional activation in human polymorphonuclear leukocytes (PMNs) is documented. This peptide suppressed the opsonized zymosan-induced generation of chemiluminescence and superoxide anion and concomitant RNA synthesis. However, this peptide did not show significant cytotoxic effects on human PMNs as judged by a cytoplasmic enzyme-releasing assay. These results indicate that a fibroblast-derived peptide reported previously has a suppressive activity on the functional activation of human PMNs. The immunological significance of this finding is discussed.
Insights
A fibroblast-derived peptide inhibits human polymorphonuclear leukocytes (PMNs) functional activation, suppressing chemiluminescence and superoxide anion generation without causing cytotoxicity. This peptide impacts immune cell responses.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- A low-molecular-weight peptide from human embryo fibroblasts (YH-1 strain) was previously identified.
- This peptide demonstrated inhibitory effects on B and T lymphocyte proliferation and function.
Purpose of the Study:
- To investigate the effect of the fibroblast-derived peptide on the functional activation of human polymorphonuclear leukocytes (PMNs).
- To determine if the peptide exhibits cytotoxic effects on PMNs.
Main Methods:
- Human PMNs were stimulated with opsonized zymosan.
- Chemiluminescence, superoxide anion generation, and RNA synthesis were measured.
- Cytotoxicity was assessed using a cytoplasmic enzyme-releasing assay.
Main Results:
- The peptide significantly suppressed opsonized zymosan-induced chemiluminescence and superoxide anion generation in PMNs.
- Concomitant suppression of RNA synthesis was observed.
- No significant cytotoxic effects on PMNs were detected.
Conclusions:
- The fibroblast-derived peptide possesses suppressive activity on the functional activation of human PMNs.
- This finding suggests a role for fibroblast-derived peptides in modulating innate immune cell responses.
- The immunological significance of this peptide's effect on PMN function warrants further investigation.