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Untargeted profiling of urinary steroid metabolites after testosterone ingestion: opening new perspectives for
Julien Boccard1, Flavia Badoud, Nicolas Jan
1School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland.
Aim:
Antidoping procedures are expected to greatly benefit from untargeted metabolomic approaches through the discovery of new biomarkers of prohibited substances abuse.
Results:
Endogenous steroid metabolites were monitored in urine samples from a controlled elimination study of testosterone undecanoate after ingestion. A platform coupling ultra-high pressure LC with high-resolution quadrupole TOF MS was used and high between-subject metabolic variability was successfully handled using a multiblock data analysis strategy. Links between specific subsets of metabolites and influential genetic polymorphisms of the UGT2B17 enzyme were highlighted.
Conclusion:
This exploratory metabolomic strategy constitutes a first step toward a better understanding of the underlying patterns driving the high interindividual variability of steroid metabolism. Promising biomarkers were selected for further targeted study.
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