Liquid microjunction surface sampling of acetaminophen, terfenadine and their metabolites in thin tissue sections
Vilmos Kertesz1, Nithya Paranthaman, Paul Moench
1Organic & Biological Mass Spectrometry Group Chemical Sciences Division, Oak Ridge National Laboratory Oak Ridge, TN 37831-6131, USA.
Background:
The aim of this work was to evaluate the analytical performance of a fully automated droplet-based surface-sampling system for determining the distribution of the drugs acetaminophen and terfenadine, and their metabolites, in rat thin tissue sections.
Results:
The rank order of acetaminophen concentration observed in tissues was stomach > small intestine > liver, while the concentrations of its glucuronide and sulfate metabolites were greatest in the liver and small intestine. Terfenadine was most concentrated in the liver and kidney, while its major metabolite, fexofenadine, was found in the liver and small intestine.
Conclusion:
The spatial distributions of both drugs and their respective metabolites observed in this work were consistent with previous studies using radiolabeled drugs.
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