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Experience with an enzyme immunoassay for serotyping human group A rotaviruses
L E Unicomb1, B S Coulson, R F Bishop
1Department of Gastroenterology, Royal Children's Hospital, Parkville, Victoria, Australia.
Journal of Clinical Microbiology
|March 1, 1989
Abstract:
An enzyme immunoassay utilizing neutralizing monoclonal antibodies to VP7 of four human group A rotavirus serotypes successfully typed rotaviruses in 71.4% (568 of 796) of fecal specimens. Sensitivity was enhanced by using homologous capture and detector antibodies. Serotyping was most successful with specimens stored for less than 3 years and containing 10(4) or more particles per ml.