[Live cell fluorescent imaging and cytotoxicity assessment of pH fluorescent probe based on styrylcyanine dyes]
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Published on: January 29, 2016
Lixia Huang1, Xiang Liu, Danting Cui
1Medical Imaging Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Objective:
To prepare a pH fluorescence probe based on styrylcyanine dyes for live cell imaging.
Methods:
The Probe 1 was prepared by reaction of 4-pyridinecarboxaldehyde with 1,1,2-trimethylbenz[e]indole. The influence of pH on the fluorescent properties was examined, and the cell viability was examined using cell counting kit-8. The Probe 1 was used as a pH fluorescence probe in living cell.
Results:
Probe 1 emitted green fluorescence under neutral and basic conditions but orange fluorescence under acid condition. Probe 1 selectively stained the cytoplasmic regions of living cells without significantly affecting the cell viability.
Conclusion:
The pH-sensitive fluorescent probe prepared based on styrylcyanine possesses good ability of cell membrane permeation for live cell fluorescent imaging.
