Expression of a novel gene encoding a 51.5 kD precursor protein is induced by different retroviral oncogenes in quail...

A cDNA clone, named T64, was isolated from a library of quail neuroretinal cells transformed by a thermosensitive v-src mutant of Rous sarcoma virus. it corresponds to the most abundant mRNA with thermodependent expression in these cells. T64 accumulation also correlated with pp60v-src activity in other cell types transformed by RSV, such as fibroblasts and myoblasts, but was independent of the proliferative state of the cells, indicating that T64 is rather implicated in the process of morphological transformation. Nuclear run on experiments showed that the accumulation of T64 mRNA in transformed neuroretinal cells is the consequence of an increased transcription rate. Enhancement of T64 expression on QNR cells was also achieved by infection with avian retroviruses harboring other oncogenes with protein kinase activity such as v-fps and v-mil. The 1.6 kb T64 mRNA was detected in vivo in a few quail tissues at levels 50-200-fold lower than in RSV-infected cells. DNA sequencing of the T64 cDNA revealed an open reading frame encoding a 449 amino acids protein with a typical N-terminal signal peptide and with significant amino acid sequence homology with a rat-secreted protein.