Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

15.4K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
15.4K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

5.0K
5.0K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

8.6K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.6K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.6K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.6K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

27.5K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
27.5K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

4.4K
4.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

iSCORE-PD: an isogenic stem cell collection to research Parkinson's disease.

Nature communications·2026
Same author

Evolving epigenomics of immune cells at single-nucleus resolution in children en route to type 1 diabetes.

Nature communications·2026
Same author

Bromodomain and extra-terminal protein inhibitors modulate natural killer cell function and differentiation.

Scientific reports·2026
Same author

ICAM-1 autoantibodies detected in healthy individuals and cross-react with functional epitopes.

ImmunoHorizons·2025
Same author

High-resolution CTCF footprinting reveals impact of chromatin state on cohesin extrusion.

Nature communications·2025
Same author

Evolving epigenomics of immune cells in type 1 diabetes at single nuclei resolution.

Research square·2025

Related Experiment Video

Updated: Apr 20, 2026

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

9.6K

SR proteins control a complex network of RNA-processing events.

Todd Bradley1, Malcolm E Cook2, Marco Blanchette3

  • 1Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas 66160, USA.

RNA (New York, N.Y.)
|November 22, 2014
PubMed
Summary
This summary is machine-generated.

SR proteins regulate gene expression by controlling alternative splicing (AS). This study reveals combinatorial control of AS by multiple SR proteins on most targets, impacting RNA processing and gene regulation.

Keywords:
RNA processingSR proteinsalternative pre-mRNA splicingregulation of gene expression

More Related Videos

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

7.7K
mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

9.7K

Related Experiment Videos

Last Updated: Apr 20, 2026

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

9.6K
MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

7.7K
mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

9.7K

Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • SR proteins are crucial for pre-mRNA alternative splicing (AS).
  • Understanding their RNA targets and binding specificity is limited.
  • General rules for SR protein AS regulation on model genes may not apply to endogenous targets.

Purpose of the Study:

  • To globally characterize AS regulation by all eight Drosophila SR protein family members.
  • To investigate SR protein binding sites and their functional diversity.
  • To explore the broader roles of SR proteins in gene expression.

Main Methods:

  • RNA sequencing (RNA-seq) to analyze global AS.
  • Individual-nucleotide resolution cross-linking and immunoprecipitation sequencing (iCLIP-seq) to map SR protein binding sites.
  • Analysis of SR protein effects on alternative promoter usage and polyadenylation.

Main Results:

  • Most AS events are regulated by multiple SR proteins, exhibiting cooperative or antagonistic control.
  • All SR proteins can promote both exon inclusion and skipping.
  • SR proteins bind diverse RNAs, including noncoding RNAs, and show positional binding around AS events.
  • SR protein levels influence alternative promoter and polyadenylation site selection.

Conclusions:

  • SR protein-mediated AS regulation is a combinatorial process involving multiple family members on most endogenous targets.
  • SR proteins play a broader role in integrating multiple layers of gene expression regulation.
  • Novel functions for SR proteins in RNA metabolism are suggested by their binding to noncoding RNAs.