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Published on: May 24, 2016
Comparison of 5'-nucleotidase activities among cultured murine melanoma cell lines
D Moody1, L J Williams, D M Gersten
1Department of Pathology, Georgetown University Medical Center, Washington, D.C. 20007.
Abstract:
The activity of 5'-nucleotidase and ouabain-sensitive Na/K ATPase was determined in seven different mouse melanoma cell lines. Ouabain-sensitive Na/K ATPase activity was found in NP40-treated cell homogenates of all cell lines. However, 5'-nucleotidase activity was found in only one mouse melanoma cell line--JB/RH. The absence of expression of 5'-nucleotidase activity in the other six cell lines is not associated with pigmentation in melanoma cells, nor is the gene switched off in all transformed melanocytes of C57BL/6 origin.
