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Related Experiment Videos

AP1/jun function is differentially induced in promotion-sensitive and resistant JB6 cells.

L R Bernstein1, N H Colburn

  • 1Johns Hopkins University, Department of Biology, Baltimore, MD 21218.

Science (New York, N.Y.)
|May 5, 1989
PubMed
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Tumor promoters activate transcription factor AP-1, crucial for neoplastic transformation. This study links AP-1 activation to promotion sensitivity in mouse JB6 cells, highlighting its role in cancer development.

Area of Science:

  • Molecular biology
  • Cancer research
  • Cell signaling

Background:

  • Tumor promoters can induce neoplastic transformation by activating specific genes.
  • The transcription factor AP-1 (activator protein 1) is implicated in this process, particularly through its activation by phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA).

Purpose of the Study:

  • To investigate the role of AP-1 activation in the promotion of neoplastic transformation.
  • To determine if AP-1 induction correlates with sensitivity to tumor promoters in mouse JB6 epidermal cells.

Main Methods:

  • Transfection of mouse JB6 epidermal cell variants (promotion-sensitive P+ and promotion-resistant P-) with a reporter construct (3XTRE-CAT) containing AP-1 cis-enhancer sequences.
  • Assay of chloramphenicol acetyltransferase (CAT) gene expression following treatment with TPA or epidermal growth factor (EGF).

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Main Results:

  • Promotion-sensitive JB6 P+ cells exhibited TPA-inducible CAT synthesis after transfection.
  • Promotion-resistant JB6 P- cells did not show inducible CAT synthesis.
  • Epidermal growth factor also induced CAT gene expression specifically in P+ cells.

Conclusions:

  • Induced AP-1 function is associated with sensitivity to tumor promoter-induced neoplastic transformation.
  • AP-1 activation is a key molecular event linking tumor promoters to cellular transformation in JB6 cells.