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Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
Published on: October 3, 2014
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Saccharides and temperature dual-responsive hydrogel layers for harvesting cell sheets
Bingbing Guo1, Guoqing Pan, Qianping Guo
1College of Chemistry, Chemical Engineering and Materials Science, Orthopedic Institute, Soochow University, 708 Renmin Road, Suzhou, Jiangsu 215007, China. yueer@suda.edu.cn binli@suda.edu.cn.
Chemical Communications (Cambridge, England)
|November 22, 2014
Summary
Researchers developed dual-responsive hydrogels for cell sheet harvesting. These hydrogels release cell sheets effectively at 37°C with increased sugar, and even better at lower temperatures and higher sugar concentrations.
Area of Science:
- Biomaterials Science
- Polymer Chemistry
- Cell Biology
Background:
- Poly(N-isopropylacrylamide) (PNIPAAm) is a well-known thermoresponsive polymer.
- Phenylboronic acid (PBA) functional groups can interact with saccharides.
- Hydrogels are crosslinked polymer networks capable of absorbing large amounts of water.
Purpose of the Study:
- To create dual-responsive hydrogels sensitive to both temperature and saccharides.
- To utilize these hydrogels for efficient cell sheet harvesting.
- To investigate the conditions for optimal cell sheet release.
Main Methods:
- Synthesis of PNIPAAm copolymers incorporating phenylboronic acid (PBA) groups.
- Preparation of hydrogels from these copolymers.
- Testing hydrogel response to temperature and varying saccharide concentrations.
- Evaluating cell sheet adhesion and release from the hydrogel surface.
Main Results:
- Successfully prepared saccharides and temperature dual-responsive hydrogels.
- Demonstrated cell sheet harvesting capabilities using the developed hydrogels.
- Showed that cell sheets can be released at 37 °C by increasing sugar concentration.
- Achieved more efficient cell sheet release at lower temperatures combined with elevated sugar concentrations.
Conclusions:
- Dual-responsive hydrogels offer a tunable platform for cell sheet engineering.
- The combination of temperature and saccharide concentration provides precise control over cell release.
- These hydrogels show promise for applications in tissue engineering and regenerative medicine.
