Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Loss of Carboxy Group as CO2: Decarboxylation of β-Ketoacids01:02

Loss of Carboxy Group as CO2: Decarboxylation of β-Ketoacids

4.2K
Carboxylic acids, upon heating, undergo a decarboxylation reaction by releasing carbon dioxide gas. Monocarboxylic acids do not undergo decarboxylation easily. However, a silver salt of carboxylic acid reacts with bromine or iodine under high temperature to release carbon dioxide gas and forms halide with one less carbon. This reaction is called the Hunsdiecker reaction.
4.2K
Carbohydrates: Dietary Sources and Requirements01:15

Carbohydrates: Dietary Sources and Requirements

2.6K
Carbohydrates are predominantly obtained from plant sources. With the exception of lactose found in milk and insignificant glycogen amounts in meat, most consumed carbohydrates have plant origins. Monosaccharides and disaccharides, or sugars, can be sourced from fruits, honey, milk, sugar cane, and sugar beets. Grains and vegetables are rich in the polysaccharide starch. Two types of polysaccharides provide fiber: cellulose, which is abundant in many vegetables, forms undigestible roughage or...
2.6K
Introduction to Seed Plants03:40

Introduction to Seed Plants

72.5K
Most plants are seed plants—characterized by seeds, pollen, and reduced gametophytes. Seed plants include gymnosperms and angiosperms.
72.5K
What are Carbohydrates?01:44

What are Carbohydrates?

205.0K
Overview
205.0K
Basic Plant Anatomy: Roots, Stems, and Leaves02:27

Basic Plant Anatomy: Roots, Stems, and Leaves

67.0K
The primary organs of vascular plants are roots, stems, and leaves, but these structures can be highly variable, adapted for the specific needs and environment of different plant species.
67.0K
Carbohydrate Digestion00:57

Carbohydrate Digestion

131.2K
Carbohydrate digestion and metabolism break down simple and complex carbohydrates from food into saccharides (i.e., sugars) for the body to use as energy. Carbohydrate digestion starts in the mouth during mastication, or chewing. The masticated carbohydrates remain intact in the stomach. Digestion resumes in the duodenum of the small intestine, where pancreatic alpha-amylase and brush border enzymes of the microvilli convert complex carbohydrates to monosaccharides. Finally, the monosaccharides...
131.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Fusarium</i> Species Infecting Greenhouse-Grown Cannabis (<i>Cannabis sativa</i>) Plants Show Potential for Mycotoxin Production in Inoculated Inflorescences and from Natural Inoculum Sources.

Journal of fungi (Basel, Switzerland)·2025
Same author

Endophytes in <i>Cannabis sativa</i>: Identifying and Characterizing Microbes with Beneficial and Detrimental Effects on Plant Health.

Plants (Basel, Switzerland)·2025
Same author

Transmission, Spread, Longevity and Management of Hop Latent Viroid, a Widespread and Destructive Pathogen Affecting Cannabis (<i>Cannabis sativa</i> L.) Plants in North America.

Plants (Basel, Switzerland)·2025
Same author

Integrated Management of Pathogens and Microbes in <i>Cannabis sativa</i> L. (Cannabis) under Greenhouse Conditions.

Plants (Basel, Switzerland)·2024
Same author

Challenges to <i>Cannabis sativa</i> Production from Pathogens and Microbes-The Role of Molecular Diagnostics and Bioinformatics.

International journal of molecular sciences·2024
Same author

Fungal and mycotoxin contaminants in cannabis and hemp flowers: implications for consumer health and directions for further research.

Frontiers in microbiology·2023

Related Experiment Video

Updated: Apr 20, 2026

In vivo and In vitro Infection of Potato Roots with Plant Parasitic Nematodes for the Assessment of Induced Structural Changes
10:35

In vivo and In vitro Infection of Potato Roots with Plant Parasitic Nematodes for the Assessment of Induced Structural Changes

Published on: February 28, 2025

1.2K

Carrot (Daucus carota L.).

Owen S D Wally1, Zamir K Punja

  • 1Department of Plant Sciences, University of Manitoba, Winnipeg, MB, Canada, R3T 2N2.

Methods in Molecular Biology (Clifton, N.J.)
|November 23, 2014
PubMed
Summary

This study details a protocol for creating transgenic carrot plants resistant to fungal pathogens. It involves transforming carrot tissues with Arabidopsis NPR1 using Agrobacterium tumefaciens and herbicide selection.

Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Biotechnology

Background:

  • Fungal pathogens pose a significant threat to plant health.
  • Enhancing plant tolerance to pathogens is crucial for agriculture.
  • Heterologous protein expression offers a strategy for disease resistance.

Purpose of the Study:

  • To describe a robust protocol for generating transgenic carrot plants.
  • To enable the expression of disease resistance genes, such as Arabidopsis NPR1, in carrots.
  • To provide a reproducible method for enhancing fungal pathogen tolerance in carrot tissues.

Main Methods:

  • Carrot petiole segments were transformed using Agrobacterium tumefaciens strain LBA4404.
  • The bar gene was used as a selectable marker for herbicide resistance.

More Related Videos

Author Spotlight: A New and Efficient Method for Comprehensive Metabolite Cytotoxicity Assessment of Triazole Pesticides in Plants
08:22

Author Spotlight: A New and Efficient Method for Comprehensive Metabolite Cytotoxicity Assessment of Triazole Pesticides in Plants

Published on: December 22, 2023

1.3K
Elucidating the Metabolism of 2,4-Dibromophenol in Plants
06:54

Elucidating the Metabolism of 2,4-Dibromophenol in Plants

Published on: February 10, 2023

1.7K

Related Experiment Videos

Last Updated: Apr 20, 2026

In vivo and In vitro Infection of Potato Roots with Plant Parasitic Nematodes for the Assessment of Induced Structural Changes
10:35

In vivo and In vitro Infection of Potato Roots with Plant Parasitic Nematodes for the Assessment of Induced Structural Changes

Published on: February 28, 2025

1.2K
Author Spotlight: A New and Efficient Method for Comprehensive Metabolite Cytotoxicity Assessment of Triazole Pesticides in Plants
08:22

Author Spotlight: A New and Efficient Method for Comprehensive Metabolite Cytotoxicity Assessment of Triazole Pesticides in Plants

Published on: December 22, 2023

1.3K
Elucidating the Metabolism of 2,4-Dibromophenol in Plants
06:54

Elucidating the Metabolism of 2,4-Dibromophenol in Plants

Published on: February 10, 2023

1.7K
  • Transgenic calli were selected and regenerated into plantlets through tissue culture.

    • Main Results:

    • The protocol successfully produced transgenic carrot plants with defined T-DNA inserts.
    • Transformation efficiency ranged from 1 to 3 Southern-positive events per 100 explants.
    • Embryogenic calli were generated and could be propagated in suspension cultures.

    Conclusions:

    • The described protocol is effective for producing transgenic carrot plants expressing beneficial genes.
    • This method provides a foundation for developing crops with enhanced resistance to fungal diseases.
    • The successful transformation opens avenues for further research in plant-pathogen interactions.