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Related Experiment Video

Updated: Apr 20, 2026

Author Spotlight: Tissue Culture-Free Agrobacterium-Mediated Transformation in Large Woody Plants
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Cassava (Manihot esculenta Crantz).

Simon E Bull1

  • 1Plant Biotechnology Group, ETH Zurich, Universitaetstrasse 2, Zurich, 8092, Switzerland, sbull@ethz.ch.

Methods in Molecular Biology (Clifton, N.J.)
|November 23, 2014
PubMed
Summary

Genetic transformation in cassava (Manihot esculenta) is crucial for crop improvement. This study details Agrobacterium-mediated transformation of friable callus to generate improved cassava varieties.

Area of Science:

  • Plant Biotechnology
  • Genetics
  • Agricultural Science

Background:

  • Genetic transformation is vital for plant research and crop improvement.
  • Advances in cassava transformation enable the development of favorable traits.
  • Agrobacterium-mediated transformation is a widely adopted method in African research labs.

Purpose of the Study:

  • To detail the Agrobacterium-mediated transformation protocol for cassava.
  • To provide supplementary notes for successful transgenic cassava production.
  • To facilitate the generation of stably transformed cassava plants with desirable traits.

Main Methods:

  • Utilizing axillary meristem tissue to produce somatic embryos and friable callus.
  • Employing Agrobacterium harboring a binary expression cassette for tissue transformation.

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  • Regenerating transformed tissue via cotyledons and shoot organogenesis to produce plantlets.

    • Main Results:

    • Successful generation of stably transformed cassava plants.
    • Detailed protocol applicable to the model cultivar 60444.
    • Established a reproducible method for producing transgenic cassava.

    Conclusions:

    • The described Agrobacterium-mediated transformation protocol is effective for cassava improvement.
    • This method supports the development of enhanced cassava varieties for agricultural applications.
    • The protocol provides a valuable resource for researchers working on cassava genetic modification.