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Grapevine (Vitis vinifera L.).

Laurent Torregrosa1, Sandrine Vialet, Angélique Adivèze

  • 1Montpellier SupAgro-INRA, UMR AGAP - Genetic Improvement and Adaptation of Mediterranean and Tropical Plants, 2 Place Pierre Viala, 34060, Montpellier Cedex, France.

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Summary

Developing efficient grapevine transformation systems is crucial for improving wine grape cultivars. This research details a reliable Agrobacterium-mediated method for genetic enhancement, preserving cultivar integrity.

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Area of Science:

  • Plant Biotechnology
  • Viticulture
  • Genetics

Background:

  • Grapevine (Vitis) is a major global fruit crop, with Vitis vinifera L. cultivars prized for premium wine production.
  • Premium wines derive from a limited number of established cultivars, making traditional breeding challenging for improvement.
  • Transgenic approaches offer precise trait introduction with minimal disruption to existing cultivar characteristics.

Purpose of the Study:

  • To describe an efficient Agrobacterium-mediated grapevine transformation system.
  • To detail protocols for producing transformed roots and semi-transgenic grafted plants.
  • To facilitate genetic improvement of wine grape cultivars.

Main Methods:

  • Establishing highly regenerative transformable grapevine tissues.
  • Optimizing co-cultivation conditions for grapevine and Agrobacterium.
  • Implementing an efficient selection regime for transgenic plant regeneration.

Main Results:

  • A reliable grapevine transformation system meeting key efficiency criteria was developed.
  • Protocols for generating transformed roots for functional gene studies were established.
  • A method for producing semi-transgenic grafted plants was successfully demonstrated.

Conclusions:

  • The described Agrobacterium-mediated transformation system is effective for grapevine improvement.
  • This system supports genetic studies and the development of enhanced wine grape cultivars.
  • The protocols enable the preservation of traditional cultivar traits while introducing new beneficial characteristics.