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Characterization of a streptomycin-sulfonamide resistance plasmid from Actinobacillus pleuropneumoniae

P J Willson1, H G Deneer, A Potter

  • 1Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada.

Antimicrobial Agents and Chemotherapy
|February 1, 1989
PubMed

Insights

A novel plasmid, pHD8.1, found in Actinobacillus pleuropneumoniae confers resistance to streptomycin and sulfonamide. This plasmid shares genetic similarities with other known resistance plasmids, aiding in understanding antimicrobial resistance.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Antimicrobial resistance is a growing global health concern.
  • Plasmids are key vectors for the spread of antibiotic resistance genes.
  • Actinobacillus pleuropneumoniae is a significant pathogen in swine.

Purpose of the Study:

  • To characterize a novel plasmid (pHD8.1) from Actinobacillus pleuropneumoniae.
  • To determine the genetic relatedness of pHD8.1 to other known plasmids.
  • To identify the genes responsible for conferring antibiotic resistance.

Main Methods:

  • Plasmid isolation and purification.
  • Restriction endonuclease mapping.
  • DNA-DNA hybridization.
  • Antimicrobial susceptibility testing.

Main Results:

  • Plasmid pHD8.1 confers resistance to streptomycin and sulfonamide.
  • pHD8.1 shows genetic relatedness to RSF1010 (Salmonella panama) and pHD148 (Haemophilus ducreyi).
  • RSF1010 also confers resistance to streptomycin and sulfonamide, while pHD148 confers only sulfonamide resistance.

Conclusions:

  • pHD8.1 represents a novel mobile genetic element carrying important resistance genes.
  • Understanding the genetic basis of resistance in Actinobacillus pleuropneumoniae is crucial for developing effective treatment strategies.
  • The relatedness of pHD8.1 to other plasmids suggests potential horizontal gene transfer mechanisms contributing to antimicrobial resistance spread.

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