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Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

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Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Published on: January 18, 2020

Osteogenic Differentiation from Embryonic Stem Cells.

Yanhong Yu¹, Carlos Pilquil¹, Michal Opas²

¹Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada, M5S 1A8.

Methods in Molecular Biology (Clifton, N.J.)

|November 24, 2014

Summary

This study details the maintenance and osteogenic differentiation of mouse embryonic stem (ES) cells. It provides a routine protocol for researchers studying cell differentiation pathways.

Keywords:

Embryoid bodies Hanging drop Murine embryonic stem cells Osteogenesis Routine culture

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Area of Science:

Stem cell biology
Developmental biology
Cellular differentiation

Background:

Embryonic stem (ES) cells possess pluripotency and self-renewal capabilities.
Murine ES cells are valuable models for exploring molecular pathways of differentiation.
Understanding differentiation is crucial for regenerative medicine and developmental studies.

Purpose of the Study:

To describe the methods for maintaining murine ES cells in culture.
To present a standard protocol for osteogenic differentiation of ES cells.
To provide a reproducible method for laboratory use.

Main Methods:

Culture of murine embryonic stem cells under specific conditions.
Induction of osteogenic differentiation using a defined protocol.
Monitoring of differentiation markers (not specified in abstract).

Main Results:

Successful maintenance of murine ES cells in culture.
Establishment of a routine protocol for osteogenic differentiation.
The protocol is suitable for laboratory application.

Conclusions:

The described methods ensure proper maintenance of murine ES cells.
The provided osteogenic differentiation protocol is effective and reproducible.
This protocol facilitates research into ES cell differentiation pathways.