Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Master Transcription Regulators

Master Transcription Regulators

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Engineered prime editors with minimal genomic errors.

Nature·2025

Same author

Mutant p53 exploits enhancers to elevate immunosuppressive chemokine expression and impair immune checkpoint inhibitors in pancreatic cancer.

Immunity·2025

Same author

RNA Dynamics Regulate Transcriptional Condensate Vivacity to Drive Gene Coordination.

bioRxiv : the preprint server for biology·2025

Same author

Mutant p53 Exploits Enhancers to Elevate Immunosuppressive Chemokine Expression and Impair Immune Checkpoint Inhibitors in Pancreatic Cancer.

bioRxiv : the preprint server for biology·2024

Same author

Engineered prime editors with minimal genomic errors.

bioRxiv : the preprint server for biology·2024

Same author

The future of open research policy should be evidence based.

Proceedings of the National Academy of Sciences of the United States of America·2024

Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026

Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026

Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026

Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026

Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026

Same journal

Systematic discovery of pathogen effector functions across human pathogens and pathways.

Cell·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 20, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Building robust transcriptomes with master splicing factors.

Mohini Jangi¹, Phillip A Sharp²

¹David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

|November 24, 2014

Summary

This summary is machine-generated.

Coherent splicing networks, involving coordinated gene splicing events, are regulated by master splicing factors. These key regulators respond to environmental cues, maintaining tissue-specific gene expression patterns during development.

More Related Videos

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Related Experiment Videos

Last Updated: Apr 20, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Area of Science:

Molecular Biology
Developmental Biology
Genetics

Background:

Gene splicing is a crucial post-transcriptional modification process.
Coherent splicing networks involve coordinated regulation of multiple splicing events.
Understanding tissue-specific gene expression is vital for developmental processes.

Purpose of the Study:

To investigate the properties of robust, context-specific splicing networks.
To propose a model for how splicing is regulated during development.
To identify the role of master splicing factors in maintaining tissue transcriptomes.

Main Methods:

Analysis of gene splicing patterns.
Computational modeling of regulatory networks.
Investigating the function of splicing regulatory proteins.

Main Results:

Identified key splicing regulators, termed "master splicing factors."
Demonstrated that these factors respond to environmental cues.
Showed their importance in establishing and maintaining tissue-specific transcriptomes.

Conclusions:

Master splicing factors play a critical role in developmental gene regulation.
Environmental cues influencing splicing are essential for tissue development.
Coherent splicing networks provide robustness to developmental processes.