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Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
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Coordinating expression of RNA binding proteins with their mRNA targets
Huifeng Jiang1, Lin Xu2, Zhe Wang2
11] Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China [2] Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, USA.
Scientific Reports
|November 25, 2014
Summary
RNA binding proteins (RBPs) co-regulate their target genes, primarily by dampening expression variations. This study reveals how RBPs, like PUF3, stabilize mRNA levels through differential decay, offering insights into gene expression coordination.
Area of Science:
- Molecular Biology
- Systems Biology
- Genetics
Background:
- Post-transcriptional regulation by RNA binding proteins (RBPs) is crucial for biological processes.
- Understanding the global regulatory network of RBPs and their mRNA targets is essential.
Purpose of the Study:
- To analyze the global regulatory relationship between RBPs and their target mRNAs in yeast.
- To investigate how RBPs influence the expression variation of their target genes.
- To elucidate the mechanisms by which RBPs coordinate gene expression.
Main Methods:
- Global analysis of RBP-mRNA regulatory relationships in yeast.
- Examination of the RBP gene PUF3 and its effect on target mRNA decay.
- Construction of a mathematical model to describe RBP-target gene expression dynamics.
Main Results:
- Most RBP genes are co-regulated with their target genes.
- RBPs tend to dampen expression variation among their target mRNAs.
- The protein PUF3 decreases target mRNA variation by differentially affecting mRNA decay.
Conclusions:
- RBPs play a significant role in coordinating the expression of their target genes.
- Dampening expression variation is a key function of RBPs.
- Mathematical modeling provides a framework for understanding RBP-mediated gene regulation.
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