Related Experiment Video
Updated: Apr 20, 2026
10:34
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
Published on: December 9, 2022
5.5K
Profiling of ribose methylations in RNA by high-throughput sequencing
Ulf Birkedal1, Mikkel Christensen-Dalsgaard, Nicolai Krogh
1Department of Cellular and Molecular Medicine, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N (Denmark).
Angewandte Chemie (International Ed. in English)
|November 25, 2014
Summary
We developed RiboMeth-seq, a new method to map ribose methylations on ribosomal RNA (rRNA). This technique reveals crucial details about RNA modifications, their order, and interactions during ribosome biogenesis.
Area of Science:
- Molecular Biology
- Biochemistry
- Genetics
Background:
- Ribose methylations are abundant chemical modifications of ribosomal RNA (rRNA).
- These modifications are essential for ribosome assembly and accurate translation.
- Studying ribose methylations has been challenging due to a lack of efficient mapping methods.
Purpose of the Study:
- To develop and apply a novel sequencing-based method for mapping ribose methylations on rRNA.
- To investigate the role and dynamics of rRNA modifications in yeast ribosomes.
- To explore the potential of this method for studying other RNA classes.
Main Methods:
- Development of RiboMeth-seq, a sequencing-based approach for detecting ribose methylations.
- Application of RiboMeth-seq to analyze yeast ribosomes.
- Utilizing the method to identify known and novel modifications, partial modifications, and inter-modification communication.
Main Results:
- RiboMeth-seq successfully detected known and new ribose methylation sites in yeast rRNA.
- The study revealed partial modifications and unexpected crosstalk between modification events.
- The order of modifications during ribosome biogenesis was determined for several sites.
- The method also provided insights into other types of RNA modifications.
Conclusions:
- RiboMeth-seq is an effective tool for detailed analysis of RNA modifications in ribosomes.
- This method enhances our understanding of the importance of RNA modifications in cellular machinery.
- RiboMeth-seq holds promise for broader applications in studying modifications in other RNA molecules, such as mRNA.
Related Concept Videos
02:24Ribosome Profiling
4.4K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.4K
03:21RNA-seq
12.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.7K