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BSA-coated nanoparticles for improved SERS-based intracellular pH sensing.

Xiao-Shan Zheng1, Pei Hu, Yan Cui

  • 1State Key Laboratory of Physical Chemistry of Solid Surfaces, ‡The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, and §Collaborative Innovation Center of Chemistry for Energy Materials, College of Chemistry and Chemical Engineering, and ∥School of Physics and Mechanical & Electrical Engineering, Xiamen University , Xiamen 361005, China.

Analytical Chemistry
|November 25, 2014
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Summary

Researchers developed a novel Au-(4-MPy)-BSA (AMB) nanosensor for precise local pH sensing in live cells. This surface-enhanced Raman spectroscopy (SERS) based sensor offers high sensitivity and biocompatibility for biological applications.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Cell Biology

Background:

  • Local microenvironment pH is crucial for understanding biological processes.
  • Surface-enhanced Raman spectroscopy (SERS) is a promising noninvasive technique for sensitive pH detection in live cells.

Purpose of the Study:

  • To develop a facile and effective pH nanosensor for intracellular pH analysis.
  • To utilize SERS for reliable and sensitive monitoring of pH in live cells.

Main Methods:

  • Fabrication of a novel Au-(4-MPy)-BSA (AMB) nanosensor.
  • Utilized 4-mercaptopyridine (4-MPy) as the pH sensing molecule.
  • Incorporated Bovine Serum Albumin (BSA) for enhanced sensitivity and biocompatibility.

Main Results:

  • The AMB nanosensor demonstrated high sensitivity to pH changes within the range of 4.0 to 9.0.
  • The nanosensor exhibited excellent biocompatibility, stability, and reliability in diverse solutions, including cell culture media.
  • Successfully applied the AMB nanosensor to monitor intracellular pH distribution in live cells.

Conclusions:

  • The developed AMB pH nanosensor offers a robust platform for reliable intracellular pH analysis.
  • This SERS-based nanosensor addresses key challenges in practical biological applications requiring precise pH monitoring.
  • The AMB nanosensor shows significant potential for advancing SERS applications in live cell studies.