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IS421, a new insertion sequence in Escherichia coli.

S Sato1, Y Nakada, A Shiratsuchi

  • 1Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.

FEBS Letters
|May 22, 1989
PubMed
Summary

Researchers identified and sequenced a new insertion sequence (IS) element, IS421, in Escherichia coli. This element has specific structural features and potential protein-coding regions, with varying copy numbers across E. coli strains.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Insertion sequence (IS) elements are mobile genetic components found in bacterial genomes.
  • Understanding IS elements is crucial for comprehending genome dynamics and evolution.

Purpose of the Study:

  • To determine the nucleotide sequence and structural characteristics of a novel IS element in Escherichia coli, designated IS421.
  • To investigate the potential protein-coding capacity and genomic distribution of IS421.

Main Methods:

  • DNA sequencing to determine the complete nucleotide sequence of IS421.
  • Bioinformatic analysis to identify open reading frames (ORFs) and terminal repeats.
  • Southern hybridization to quantify the copy number of IS421 in different E. coli strains.

Main Results:

  • IS421 is 1340 bp long with 22 bp inverted terminal repeats and is flanked by 13 bp direct repeats.
  • Two ORFs were identified: one encoding a 371 amino acid (aa) polypeptide with potential transposase homology, and another encoding a 102 aa polypeptide.
  • IS421 copy numbers were 4 in E. coli K-12 and B, and 5 in E. coli C.

Conclusions:

  • IS421 represents a newly characterized insertion sequence element in Escherichia coli.
  • Its structural features suggest potential mobility and functional activity, possibly related to transposition.
  • The varying copy numbers indicate differential distribution and potential integration events across E. coli strains.

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