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Related Experiment Videos

How do inositol phosphates regulate calcium signaling?

J W Putney1, H Takemura, A R Hughes

  • 1Laboratory of Cellular and Molecular Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|June 1, 1989
PubMed
Summary

Cell surface receptor activation triggers phosphatidylinositol 4,5-bisphosphate hydrolysis, generating signaling molecules. Inositol 1,4,5-trisphosphate releases intracellular calcium, with its action terminated by metabolism.

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Area of Science:

  • Cellular signaling pathways
  • Molecular biology
  • Biochemistry

Background:

  • Cell surface receptor activation initiates signal transduction cascades.
  • Phospholipase C hydrolyzes phosphatidylinositol 4,5-bisphosphate, producing key second messengers.
  • Inositol 1,4,5-trisphosphate (InsP3) is a critical mediator of intracellular calcium release.

Purpose of the Study:

  • To elucidate the mechanisms of calcium mobilization and entry following cell surface receptor activation.
  • To investigate the metabolic pathways terminating the action of inositol 1,4,5-trisphosphate.
  • To explore the role of InsP3 in secondary calcium influx.

Main Methods:

  • Investigating phospholipase C-catalyzed hydrolysis of phosphatidylinositol 4,5-bisphosphate.

Related Experiment Videos

  • Studying the metabolism of inositol 1,4,5-trisphosphate via 5-phosphatase and 3-kinase pathways.
  • Analyzing calcium release from intracellular stores stimulated by InsP3.

    • Main Results:

    • Receptor activation leads to InsP3 formation and subsequent intracellular calcium release.
    • InsP3 metabolism occurs via dephosphorylation to inositol 1,4-bisphosphate or phosphorylation to inositol 1,3,4,5-tetrakisphosphate.
    • The precise mechanisms of calcium entry remain unclear, with a proposed model involving InsP3-mediated emptying of intracellular calcium pools.

    Conclusions:

    • Inositol 1,4,5-trisphosphate is a key mediator of calcium release from intracellular stores.
    • Metabolic inactivation of InsP3 is crucial for signal termination.
    • Further research is needed to fully understand the mechanisms governing calcium entry downstream of InsP3 signaling.