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Three-Dimensional Microscopy in Microbiology

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Related Experiment Video

Updated: Apr 20, 2026

Autofluorescence Imaging to Evaluate Red Algae Physiology

Autofluorescence Imaging to Evaluate Red Algae Physiology

Published on: February 17, 2023

Imaging nanoparticle-algae interactions in three dimensions using Cytoviva microscopy.

P Vallotton¹, B Angel, M McCall

¹CSIRO - Digital Productivity & Services, Locked Bag 17, North Ryde, New South Wales, 1670, Australia.

Journal of Microscopy

|November 26, 2014

Summary

This summary is machine-generated.

This study introduces a 3D imaging method for nanoparticles using an enhanced Cytoviva microscopy platform. This technique allows detailed visualization of nanoparticle interactions with algal cells in their complete three-dimensional environment.

Keywords:

Cytoviva Three-dimensional visualization algae dark field nanoparticles nanotoxicity subcapitata

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Area of Science:

Nanotechnology
Environmental Science
Microscopy

Background:

Metal nanoparticles offer ultrasensitive imaging for biological assays.
The Cytoviva microscopy platform detects nanoscale particles but is limited to single-plane imaging.
Existing methods provide only partial sample views, hindering detailed analysis.

Purpose of the Study:

To develop a 3D imaging technique for nanoparticles.
To overcome the single-plane limitation of Cytoviva microscopy.
To visualize nanoparticle-cell interactions in full 3D context.

Main Methods:

Mounting the Cytoviva condenser on an automated microscope with Z-scanning capability.
Developing a method for three-dimensional mapping of nanoparticles.
Applying the technique to study silver and cerium dioxide nanoparticles with Pseudokirchneriella subcapitata cells.

Main Results:

Achieved 3D mapping of nanoparticles within their cellular environment.
Enabled detailed visualization of nanoparticle-cell interactions.
Facilitated determination of nanoparticle location relative to cells (inside, surface, or external).

Conclusions:

The developed 3D imaging method overcomes Cytoviva's limitations.
This technique provides comprehensive insights into nanoparticle-cell interactions.
It is applicable to environmentally relevant systems like algae and various nanoparticles.