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Updated: Apr 20, 2026

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
Published on: March 14, 2019
Expression and clinical significance of miRNA-34a in colorectal cancer
Zhi-Bin Ma1, Xiao-Lin Kong, Gang Cui
1Department of Gastroenterology, the First Affiliated Hospital of Harbin Medical University, Harbin, China
Background:
The aim of this study was to investigate differences of miRNA-34a expression in benign and malignant colorectal lesions.
Materials And Methods:
Samples of cancer, paraneoplastic tissues and polyps were selected and total RNA was extracted by conventional methods for real-time PCR to detect the miRNA- 34a expression. In addition, the LOVO colorectal cancer cell line was cultured, treated with the demethylating agent 5-azacytidine and screened for differentially expressed miRNA-34a.
Results:
After the drug treatment, the miRNA-34a expression of colorectal cancer cell line LOVO was increased and real-time PCR showed that levels of expression in both cell line and colorectal cancer tissues were low, as compared to paraneoplastic tissue (p<0.05). Polyps tissues had significantly higher expression than paraneoplastic and colorectal cancer samples (p<0.05).
Conclusions:
miRNA-34a-5p may play a role as a tumor suppressor gene in colorectal cancer, with involvement of DNA methylation.
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