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Related Concept Videos

Complement System01:27

Complement System

13.2K
The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
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Hypersensitivity Reactions: Immune-Complex Reactions01:19

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Type III hypersensitivity reactions occur when antigen–antibody complexes form and activate the complement system. Normally, these complexes help the clearance of antigens by phagocytes and red blood cells. However, when large numbers of immune complexes are present, they can deposit in tissues—particularly in the walls of blood vessels—leading to inflammation and tissue injury. These deposits trigger complement activation and neutrophil recruitment, resulting in serum...
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Hypersensitivity Reactions: Cytolytic Reactions01:01

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Type II hypersensitivity involves IgG and IgM antibodies targeting cell surface antigens, leading to cell destruction. This can occur through complement activation, antibody-dependent cell-mediated cytotoxicity (ADCC), or acting as opsonins for phagocytosis. When excessive, these reactions cause significant tissue damage.Drug-induced hemolytic anemia is a common example, where drugs like penicillin or cephalosporins bind to red blood cells, forming drug-protein complexes. These complexes...
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Depletion of Specific Cell Populations by Complement Depletion
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Immunosuppressive Effect of Hypodermin C on Complement Component 3 In Vitro.

An-Kang Hu1,2, Ren-Jin Chen2, Xiao-Rong Zhu2

  • 1College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China.

Cell Biochemistry and Biophysics
|November 27, 2014
PubMed
Summary
This summary is machine-generated.

Hypodermins C (HC) proteases from Hypoderma lineatum larvae degrade complement C3, inhibiting the host immune response. This suggests recombinant HC could prevent xenotransplant rejection.

Keywords:
Complement C3Hypodermin CXenogeneic rejection

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Area of Science:

  • Immunology
  • Parasitology
  • Molecular Biology

Background:

  • Hypodermins A, B, and C are proteases secreted by Hypoderma lineatum larvae.
  • These proteases play roles in larval migration and immune evasion.
  • Previous research indicated recombinant Hypodermin A has immunosuppressive properties.

Purpose of the Study:

  • To clone and characterize Hypodermins C (HC).
  • To investigate the immunosuppressive potential of recombinant HC.
  • To explore HC's role in inhibiting the complement pathway.

Main Methods:

  • Cloning of HC cDNA and transfection into Cos7 cells.
  • Assessing Cos7 cell resistance to guinea pig C3 lysis.
  • In vitro degradation assays of guinea pig C3 by HC.
  • Electrophoretic mobility shift assay to identify HC DNA binding sites.

Main Results:

  • Cos7 cells stably expressing HC showed increased resistance to C3-mediated lysis.
  • Recombinant HC protease degraded guinea pig C3 in vitro.
  • HC was shown to inhibit the complement pathway.
  • DNA binding sites of HC were identified.

Conclusions:

  • Recombinant Hypodermins C (HC) effectively degrades complement C3 and inhibits the complement pathway.
  • HC exhibits immunosuppressive properties relevant to xenotransplantation.
  • Recombinant HC shows potential as an immunosuppressive agent to prevent xenotransplant rejection.