Related Experiment Video
Updated: Apr 20, 2026

Modeling Paracrine Noncanonical Wnt Signaling In Vitro
Published on: December 10, 2021
DACT1 is involved in human placenta development by promoting Wnt signaling
Jian Hou1, Yu-Hua Wen, Kang-Ni Feng
1Key Laboratory of Regenerative Biology, South China Institute of Stem Cell and Regenerative Medicine, Institute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, China.
Objective:
The aim of this study was to evaluate the expression of DACT1 in human placenta tissue and the relationship between DACT1 and target genes of the Wnt signaling pathway.
Method:
Real-time PCR and western blotting were used to detect the expression of DACT1 and the target genes of Wnt signaling pathway in human placenta tissue. And the relationship between them was analyzed using SPSS 19.
Results:
Real-time PCR results showed that DACT1 expression was significantly higher in 49- to 71-day placenta tissues (mean value = 0.020) than that in 39- to 48-day (the mean value = 0.009). The mRNA expressions of the Wnt signaling pathway target genes, CCND1, CCND2, FOSL1, DAB2 and JUN, were also increased expressed in human placenta tissues. Significant positive associations between DACT1 and CCND1, CCND2, FOSL1, DAB2 and JUN were observed. Western blotting analysis showed that the protein expression of DACT1, CCND1, CCND2, FOSL1, DAB2 and JUN displayed the increasing trend in 43-, 49- and 71-day placenta samples.
Conclusion:
DACT1 might play an important role in human placenta development via promoting Wnt signaling.
Related Concept Videos
Canonical Wnt Signaling Pathway
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathways
Non-Canonical Wnt Signaling Pathways
Role Of Notch Signalling In Intestinal Stem Cell Renewal
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Hedgehog Signaling Pathway

