A WS2 nanosheet-based platform for fluorescent DNA detection via PNA-DNA hybridization

The WS2 nanosheet, a two-dimensional layered nanomaterial, shows high fluorescence quenching ability for the dye-labeled ssDNA. Currently, most of the fluorescent DNA detection methods employ DNA as a probe for recognition of target DNA. Peptide nucleic acid (PNA) is a DNA mimic but a neutral molecule, showing superior hybridization properties to target DNA. Based on the unique properties of WS2 nanosheet and PNA-DNA hybridization, we have developed a rapid, simple, stable and sensitive approach for DNA detection based on good fluorescence quenching ability of the WS2 nanosheet as well as high binding affinity and specificity of PNA to DNA. This novel assay is capable of exhibiting high sensitivity and specificity with a detection limit of 500 pM, and discriminating between single bases.