Related Experiment Video
Updated: Apr 20, 2026
08:40
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Published on: April 8, 2016
13.6K
Overview of automated immunohistochemistry
1From the Department of Laboratory Medicine, Geisinger Medical Center, Danville, Pennsylvania.
Archives of Pathology & Laboratory Medicine
|November 27, 2014
Summary
Automated immunohistochemistry platforms offer solutions for clinical diagnostics amid staff shortages. Understanding platform differences is key to optimizing testing volume, turnaround time, and labor savings in histology labs.
Area of Science:
- Histopathology
- Clinical Diagnostics
- Laboratory Automation
Background:
- Increasing demand for immunohistochemistry (IHC) in clinical diagnostics.
- Shortage of skilled staff in histology laboratories necessitates automation.
- Current automated IHC platforms exhibit diverse designs and capabilities.
Purpose of the Study:
- To review technological applications in automating immunohistochemical staining.
- To analyze the evolution and impact of automated IHC staining.
Main Methods:
- Comprehensive literature review of automated IHC technologies.
- Interviews with vendors of automated staining platforms.
- Analysis of personal practical experience with IHC instrumentation.
Main Results:
- Commercially available automated IHC platforms possess distinct strategic design features.
- Each platform presents unique advantages and disadvantages in workflow integration.
- Variability exists in automation capabilities impacting standardization and efficiency.
Conclusions:
- Understanding design differences in automated IHC platforms is crucial.
- Matching platform capabilities to specific laboratory demands (volume, turnaround time, labor) is essential for optimal utilization.
- Strategic selection of automated instrumentation can enhance diagnostic efficiency and consistency.
Related Concept Videos
01:22Immunocytochemistry and Immunohistochemistry
15.6K
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
These...
15.6K
01:20Immunogold Electron Microscopy
6.1K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
6.1K