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Updated: Apr 20, 2026

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
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Polymerization-based signal amplification for paper-based immunoassays.

Abraham K Badu-Tawiah1, Shefali Lathwal, Kaja Kaastrup

  • 1Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA. gwhitesides@gmwgroup.harvard.edu.

Lab on a Chip
|November 27, 2014
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Summary
This summary is machine-generated.

This study presents a new, affordable colorimetric diagnostic test for resource-limited settings. The paper-based assay uses visible light to detect Plasmodium falciparum proteins, offering clear visual results and quantitative data with cellphone imaging.

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Area of Science:

  • Biomedical Diagnostics
  • Point-of-Care Testing
  • Materials Science

Background:

  • Diagnostic tests for resource-limited settings need to be affordable, user-friendly, and require minimal infrastructure.
  • Colorimetric detection methods offer utility due to their visual readability without specialized equipment.

Purpose of the Study:

  • To develop an affordable and easy-to-use colorimetric diagnostic method for resource-limited settings.
  • To integrate paper-based immunoassay with visible-light-induced polymerization for enhanced visual contrast.

Main Methods:

  • A novel colorimetric method combining paper-based immunoassay with visible-light-induced polymerization.
  • Demonstration using Plasmodium falciparum histidine-rich protein 2 detection in human serum.
  • Integration with cellphone-based imaging for quantitative analysis.

Main Results:

  • High visual contrast achieved between positive and negative results.
  • Successful visual detection of Plasmodium falciparum histidine-rich protein 2 in complex biological matrices.
  • Quantitative detection of analyte levels possible with cellphone imaging.

Conclusions:

  • The developed method is suitable for visual detection in resource-limited settings.
  • The assay provides quantitative data when coupled with cellphone imaging.
  • The method allows for decoupling of analyte capture from signal amplification and visualization.