[Effect of vitrification on the functioning of the structural elements of the cytoskeleton in porcine oocytes]

On the basis of inhibition analysis using inhibitors of protein kinase A and polymerization of microfilament under the influence oftheophylline, GDP and GTP have studied the release of Ca2+ from intracellular stores (ryanodin- and inositol-1 ,4,5-trisphosphate) of native and devitrified porcine oocytes. It is found that theophylline, as well as GDP stimulate the release of Ca2+ from intracellular stores in the native and devitrified oocytes, but a further release of Ca2+ from intracellular stores under the joint action of theophylline and GDP was observed only in native oocytes. Inhibitors of protein kinase A and polymerization of microfilaments in native oocytes have a negative impact on the further release of Ca2+ from intracellular stores under the joint action of theophylline and GDP. In possible transition of Ca2+ between intracellular depots, that stimulated by GDF, but GTF doesn't take part. The received results indicate that disruption of the functioning of the system of microfilaments in oocytes determined by vitrification that assumes a priori difference in the signal transduction pathways in native and devitrified oocytes at diplotene stage.