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Nitric Oxide Signaling Pathway

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...

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Updated: Apr 20, 2026

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Published on: October 19, 2015

Nitric oxide donor superparamagnetic iron oxide nanoparticles.

Miguel M Molina¹, Amedea B Seabra², Marcelo G de Oliveira³

¹Instituto de Física, Universidade de São Paulo, São Paulo, São Paulo, 05508-090, Brazil.

Materials Science & Engineering. C, Materials for Biological Applications

|November 28, 2014

Summary

Researchers developed novel magnetic nanoparticles (MNPs) for targeted nitric oxide (NO) delivery. These superparamagnetic nanoparticles release therapeutic NO levels, showing significant potential for biomedical applications.

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Area of Science:

Materials Science
Biomedical Engineering
Nanotechnology

Background:

Nitric oxide (NO) plays crucial roles in various physiological processes.
Efficient and targeted delivery of NO remains a challenge in biomedical applications.

Purpose of the Study:

To develop a novel nitric oxide (NO) delivery system using magnetic nanoparticles (MNPs).
To characterize the synthesized nanoparticles and evaluate their NO releasing capabilities.

Main Methods:

Water-soluble magnetic nanoparticles (MNPs) were synthesized via co-precipitation and coated with mercaptosuccinic acid (MSA).
Nanoparticles were characterized using FTIR, XRD, TEM, and VSM.
Free thiol groups were nitrosated to create NO-releasing nanoparticles (SNO-NPs).
Nitric oxide release was quantified using chemiluminescence.

Main Results:

Synthesized SH-NPs exhibited superparamagnetic behavior at room temperature with a mean diameter of 10nm.
SNO-NPs demonstrated spontaneous release of NO in aqueous solutions.
The released NO levels were suitable for biomedical applications.

Conclusions:

A new magnetic nanoparticle-based NO delivery vehicle was successfully developed.
The SNO-NPs offer a promising platform for targeted NO therapy.
This strategy holds potential for generating desired NO amounts at specific locations.