Related Experiment Video
Updated: Apr 20, 2026
Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture
Published on: October 28, 2022
Novel preparation of controlled porosity particle/fibre loaded scaffolds using a hybrid micro-fluidic and
Maryam Parhizkar1, Panagiotis Sofokleous, Eleanor Stride
1Department of Mechanical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK.
Abstract:
The purpose of this research was to produce multi-dimensional scaffolds containing biocompatible particles and fibres. To achieve this, two techniques were combined and used: T-Junction microfluidics and electrohydrodynamic (EHD) processing. The former was used to form layers of monodispersed bovine serum albumin (BSA) bubbles, which upon drying formed porous scaffolds. By altering the T-Junction processing parameters, bubbles with different diameters were produced and hence the scaffold porosity could be controlled. EHD processing was used to spray or spin poly(lactic-co-glycolic) (PLGA), polymethysilsesquioxane (PMSQ) and collagen particles/fibres onto the scaffolds during their production and after drying. As a result, multifunctional BSA scaffolds with controlled porosity containing PLGA, PMSQ and collagen particles/fibres were obtained. Product morphology was studied by optical and scanning electron microscopy. These products have potential applications in many advanced biomedical, pharmaceutical and cosmetic fields e.g. bone regeneration, drug delivery, cosmetic cream lathers, facial scrubbing creams etc.
