Related Experiment Video
Updated: Apr 20, 2026

Bergmeyer Glucose Quantification for Microbiological Samples
Published on: January 17, 2025
Enzyme distribution and secondary structure of sol-gel immobilized glucose oxidase by micro-attenuated total
I Delfino1, M Portaccio2, B Della Ventura2
1Biophysics and Nanoscience Centre, DEB, Università della Tuscia, Viterbo, Italy.
Abstract:
Glucose oxidase (GOD) immobilized into sol-gel matrices was studied by using Micro-Attenuated Total Reflection Fourier Transform Infrared (micro-ATR FT-IR) spectroscopy in order to characterize enzyme distribution and secondary structure in systems with valuable potentialities in amperometric and optical biosensing. Spectra were acquired in the 4000-600 cm(-1) frequency region and the analysis of specific fingerprints in the FT-IR spectra evidenced that the enzyme was actually immobilized in the matrix. The enzyme spatial distribution was obtained by examining the amide I and amide II band region of spectra from defined sample positions. The deconvolution of the amide I band in terms of lorentzian functions provided information on the secondary structure of the immobilized GOD. By this approach a macroscopic preservation of GOD activity upon immobilization was evidenced along with the existence of some matrix sites with locally inactivated GOD. To our knowledge this is the first example of point-by-point characterization of conformational changes of immobilized enzyme by means of micro-ATR infrared spectroscopy, thus confirming that this technique can be usefully employed for a non- or minimally-invasive detailed micro-characterization of catalytic supports in order to improve their functionality.
More Related Videos
11:38In situ FTIR Spectroscopy as a Tool for Investigation of Gas/Solid Interaction: Water-Enhanced CO2 Adsorption in UiO-66 Metal-Organic Framework
Published on: February 1, 2020
11:44Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
Related Concept Videos
IR and UV–Vis Spectroscopy of Aldehydes and Ketones
UV–Vis Spectroscopy of Conjugated Systems
One of the factors influencing λmax is...
IR and UV–Vis Spectroscopy of Carboxylic Acids
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency,...
UV–Vis Spectroscopy: Beer–Lambert Law
IR Frequency Region: Fingerprint Region