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Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
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IMP-1 encoded by a novel Tn402-like class 1 integron in clinical Achromobacter xylosoxidans, China

Zhenhong Chen1, Haihong Fang2, Li Wang1

  • 1Nanlou Respiratory Diseases Department, Chinese People's Liberation Army General Hospital, Beijing 100853, China.

Scientific Reports
|November 28, 2014
PubMed

Insights

Achromobacter xylosoxidans strain A22732 from China produces OXA-114e and IMP-1 carbapenemases, conferring resistance to multiple beta-lactam antibiotics. This study reports the first carbapenemase-encoding IncP-1ß plasmid in a clinical isolate.

Area of Science:

  • Microbiology
  • Genetics
  • Infectious Diseases

Background:

  • Achromobacter xylosoxidans is an opportunistic pathogen.
  • Carbapenem resistance is a growing global health concern.
  • Carbapenemases are enzymes that degrade carbapenem antibiotics.

Purpose of the Study:

  • To characterize the carbapenem resistance mechanisms in Achromobacter xylosoxidans strain A22732.
  • To identify the genetic elements responsible for carbapenemase production.
  • To investigate the plasmid-mediated spread of carbapenem resistance.

Main Methods:

  • Whole-genome sequencing
  • Plasmid analysis
  • Conjugation experiments
  • Antimicrobial susceptibility testing

Main Results:

  • Achromobacter xylosoxidans strain A22732 produces OXA-114e (chromosomal) and IMP-1 (plasmid-borne) carbapenemases.
  • The blaIMP-1 gene is part of a novel Tn402-like class 1 integron on a conjugative IncP-1ß plasmid.
  • The plasmid also carries aacA7 and orfE genes.
  • A blaIMP-1 gene cassette-specific promoter was identified in addition to the intrinsic PcW promoter.

Conclusions:

  • This is the first report of a carbapenemase-encoding IncP-1ß plasmid in a clinical Achromobacter xylosoxidans isolate.
  • The IncP-1ß plasmid carrying blaIMP-1 represents a significant threat for the dissemination of carbapenem resistance.
  • Understanding the genetic basis of carbapenem resistance is crucial for developing effective treatment strategies.

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