Related Experiment Video
Updated: Apr 20, 2026
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
Published on: February 27, 2020
LuciPHOr2: site localization of generic post-translational modifications from tandem mass spectrometry data
Damian Fermin1, Dmitry Avtonomov1, Hyungwon Choi1
1Department of Pathology, University of Michigan Medical School, Ann Arbor, MI, USA, Saw Swee Hock School of Public Health, National University of Singapore and National University Health System, Singapore, Singapore and Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, USA.
Unlabelled:
We present LuciPHOr2, a site localization tool for generic post-translational modifications (PTMs) using tandem mass spectrometry data. As an extension of the original LuciPHOr (version 1) for phosphorylation site localization, the new software provides a site-level localization score for generic PTMs and associated false discovery rate called the false localization rate. We describe several novel features such as operating system independence and reduced computation time through multiple threading. We also discuss optimal parameters for different types of data and illustrate the new tool on a human skeletal muscle dataset for lysine-acetylation.
Availability And Implementation:
The software is freely available on the SourceForge website http://luciphor2.sourceforge.net.
Supplementary Information:
Supplementary data are available at Bioinformatics online.
