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Updated: Apr 20, 2026

Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders
Published on: December 5, 2016
First-generation linkage map for the European tree frog (Hyla arborea) with utility in congeneric species
Christophe Dufresnes1, Alan Brelsford, Nicolas Perrin
1Department of Ecology and Evolution, Biophore building, University of Lausanne, 1015 Lausanne, Switzerland. Christophe.Dufresnes@unil.ch.
Background:
Western Palearctic tree frogs (Hyla arborea group) represent a strong potential for evolutionary and conservation genetic research, so far underexploited due to limited molecular resources. New microsatellite markers have recently been developed for Hyla arborea, with high cross-species utility across the entire circum-Mediterranean radiation. Here we conduct sibship analyses to map available markers for use in future population genetic applications.
Findings:
We characterized eight linkage groups, including one sex-linked, all showing drastically reduced recombination in males compared to females, as previously documented in this species. Mapping of the new 15 markers to the ~200 My diverged Xenopus tropicalis genome suggests a generally conserved synteny with only one confirmed major chromosome rearrangement.
Conclusions:
The new microsatellites are representative of several chromosomes of H. arborea that are likely to be conserved across closely-related species. Our linkage map provides an important resource for genetic research in European Hylids, notably for studies of speciation, genome evolution and conservation.
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