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Related Experiment Video

Updated: Apr 20, 2026

Visualizing Shifts on Neuron-Glia Circuit with the Calcium Imaging Technique
11:41

Visualizing Shifts on Neuron-Glia Circuit with the Calcium Imaging Technique

Published on: April 8, 2022

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Calcium imaging in neuron cell death.

María Calvo1, Carlos Villalobos, Lucía Núñez

  • 1Instituto de Biología y Genética Molecular (IBGM), Consejo Superior de Investigaciones Científicas y Universidad de Valladolid, c/ Sanz y Forés 3, Valladolid, 47003, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|November 29, 2014
PubMed
Summary

Monitoring intracellular calcium (Ca2+) levels and apoptosis in neurons is crucial for understanding cell death. This study presents a method to simultaneously measure cytosolic calcium concentration and apoptosis in the same rat hippocampal neurons.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Intracellular calcium (Ca2+) regulates vital cell functions, including apoptosis and neuron death.
  • Elevated intracellular Ca2+ levels are implicated in excitotoxicity-induced neuron death.

Purpose of the Study:

  • To establish a method for simultaneously monitoring intracellular Ca2+ concentration and apoptosis in individual neurons.
  • To facilitate research into the role of Ca2+ in neuron cell death.

Main Methods:

  • Primary cultures of hippocampal rat neurons were prepared.
  • Fluorescence imaging using Fura-2 AM was employed to measure cytosolic Ca2+ concentration ([Ca2+]cyt).
  • Apoptosis was detected using immunofluorescence imaging.

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Last Updated: Apr 20, 2026

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Subcellular Imaging of Neuronal Calcium Handling In Vivo
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Main Results:

  • A straightforward protocol for the concurrent measurement of [Ca2+]cyt and apoptosis in the same neurons was developed.
  • This method allows for direct correlation between Ca2+ dynamics and apoptotic markers.

Conclusions:

  • The described method enables simultaneous assessment of Ca2+ levels and apoptosis in neurons.
  • This technique is valuable for investigating Ca2+-mediated cell death mechanisms in neuroscience research.