Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Embryonic Stem Cells00:58

Embryonic Stem Cells

33.6K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
33.6K
Embryonic Stem Cells00:57

Embryonic Stem Cells

6.0K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
6.0K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.7K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.7K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

6.4K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
6.4K
Stem Cell Culture01:17

Stem Cell Culture

6.7K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
6.7K
Source And Potency Of Stem Cells01:27

Source And Potency Of Stem Cells

6.9K
Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
6.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial morphology in human fibroblasts and induced pluripotent stem cells in Leigh syndrome: A comparative analysis.

Physiological reports·2026
Same author

What Patients Call Gynaecological Conditions: A Qualitative Study.

BJOG : an international journal of obstetrics and gynaecology·2026
Same author

Investigating the impact of multinational collaborations on cultural understanding, health disparities, biomedical innovations, and professional development through project-based learning.

Journal of biological engineering·2026
Same author

Impaired mitochondrial morphology and respiratory dysfunction in human induced pluripotent stem cells with mitochondrial tRNA mutations (m.3243A>G and m.14739G>A).

Orphanet journal of rare diseases·2026
Same author

Intervention Counseling for Return to Sex After Urogynecologic Surgery: A Randomized Controlled Trial.

Obstetrics and gynecology·2025
Same author

Standardized Counseling Tool for Returning to Sexual Activity After Pelvic Reconstructive Surgery.

Obstetrics and gynecology·2025

Related Experiment Video

Updated: Apr 20, 2026

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
12:06

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

Published on: January 28, 2015

13.1K

Stem cells, neural progenitors, and engineered stem cells.

Raj R Rao1, Shilpa Iyer

  • 1Department of Chemical and Life Science Engineering, Virginia Commonwealth University, 601 West Main Street, Room 410, Richmond, VA, 23284-3028, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 29, 2014
PubMed
Summary
This summary is machine-generated.

Human pluripotent stem cells can generate neural progenitors for disease modeling. Novel mitochondrial transfection technology enables engineering these cells for studying neuro-mitochondrial disorders in vitro.

More Related Videos

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
12:13

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Published on: July 11, 2019

7.8K
Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
13:58

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

Published on: July 29, 2015

16.2K

Related Experiment Videos

Last Updated: Apr 20, 2026

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
12:06

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

Published on: January 28, 2015

13.1K
Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
12:13

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Published on: July 11, 2019

7.8K
Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
13:58

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

Published on: July 29, 2015

16.2K

Area of Science:

  • Stem cell biology
  • Neuroscience
  • Mitochondrial biology

Background:

  • Human pluripotent stem cells (hPSCs) differentiate into all human cell types.
  • hPSCs offer potential for regenerative medicine and disease modeling.
  • Engineering stem cells is crucial for creating accurate in vitro disease models.

Purpose of the Study:

  • To detail the propagation and characterization of human neural progenitors (hNPs) from hPSCs.
  • To focus on engineering hNPs for in vitro disease modeling of human neuro-mitochondrial disorders.
  • To present protocols for novel mitochondrial transfection technology in hNPs.

Main Methods:

  • Culture and characterization of hPSCs and hNPs.
  • Engineering hNPs using a novel mitochondrial transfection technique.
  • Development of in vitro disease models for neuro-mitochondrial disorders.

Main Results:

  • Established protocols for hPSC and hNP culture and characterization.
  • Successfully engineered hNPs using the novel mitochondrial transfection technology.
  • Demonstrated the potential for creating in vitro models of human neuro-mitochondrial disorders.

Conclusions:

  • hPSCs are a valuable source for generating hNPs.
  • Novel mitochondrial transfection technology enables precise engineering of hNPs.
  • Engineered hNPs provide a robust platform for studying neuro-mitochondrial disorders.