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High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics
Nobuaki Takemori1, Ayako Takemori, Kazuhiro Matsuoka
1Proteo-Science Center, Ehime University, Ehime, Japan. takemori@m.ehime-u.ac.jp.
Molecular Biosystems
|November 29, 2014
Abstract:
Using a wheat germ cell-free protein synthesis system, we developed a high-throughput method for the synthesis of stable isotope-labeled full-length transmembrane proteins as proteoliposomes to mimic the in vivo environment, and we successfully constructed an internal standard library for targeted transmembrane proteomics by using mass spectrometry.

