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NAIF1 inhibits gastric cancer cells migration and invasion via the MAPK pathways

Mei Yang1, Yu-Yu Gu, Hua Peng

  • 1Department of Etiology and Carcinogenesis and State Key Laboratory of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 17 Panjiayuan Nanli, Chaoyang District, Beijing, 100021, People's Republic of China.

Journal of Cancer Research and Clinical Oncology
|November 30, 2014
PubMed
Abstract

Insights

Nuclear apoptosis-inducing factor 1 (NAIF1) inhibits gastric cancer cell proliferation, migration, and invasion. NAIF1 shows potential as a therapeutic target for gastric cancer by affecting key signaling pathways.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cell Biology

Background:

  • Nuclear apoptosis-inducing factor 1 (NAIF1) expression is reduced in gastric cancer.
  • The precise role of NAIF1 in gastric cancer progression remains unclear.
  • NAIF1 has shown potential to induce apoptosis in gastric cancer cells.

Purpose of the Study:

  • To investigate the functional role of NAIF1 in gastric cancer cells.
  • To elucidate the molecular mechanisms underlying NAIF1's effects on gastric cancer.
  • To evaluate NAIF1 as a potential therapeutic target for gastric cancer.

Main Methods:

  • Cell viability assessed using MTT and colony formation assays.
  • Cell migration and invasion analyzed via transwell assays.
  • Protein and RNA expression levels determined by Western blot and qPCR, respectively.
  • In vivo anti-tumor efficacy evaluated in a xenograft mouse model.

Main Results:

  • NAIF1 overexpression suppressed proliferation, migration, and invasion in gastric cancer cell lines (BGC823, MKN45).
  • NAIF1 reduced tumor growth in a xenograft model.
  • NAIF1 downregulated MMP2, MMP9, FAK activation, JNK, and ERK1/2 expression.
  • NAIF1 promoted JNK degradation via the ubiquitin-proteasome pathway.

Conclusions:

  • NAIF1 regulates gastric cancer cell migration and invasion.
  • NAIF1 impacts cellular processes through the MAPK signaling pathway.
  • NAIF1 represents a promising therapeutic target for gastric cancer treatment.

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