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Involvement of p29/SYF2/fSAP29/NTC31 in the progression of NSCLC via modulating cell proliferation

Yifei Liu1, Tingting Ni2, Qun Xue3

  • 1Department of Pathology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China.

Pathology, Research and Practice
|December 1, 2014
PubMed

Insights

The protein p29 is elevated in non-small cell lung cancer (NSCLC) and promotes tumor growth by increasing cell proliferation. Targeting p29 may offer a new therapeutic strategy for NSCLC patients.

Area of Science:

  • Molecular Biology
  • Oncology

Background:

  • p29 (SYF2/fSAP29/NTC31) is a chromatin-associated protein involved in DNA damage response, cell cycle arrest, and pre-mRNA splicing.
  • p29 depletion leads to reduced DNA replication and increased G1 phase cell population.

Purpose of the Study:

  • To investigate the role of p29 in the regulation of non-small cell lung cancer (NSCLC) progression.

Main Methods:

  • Western blot and immunohistochemistry to analyze p29 expression in NSCLC tissues.
  • Kaplan-Meier survival analysis.
  • Small interfering RNA (siRNA) to interfere with p29 expression in A549 cells.

Main Results:

  • p29 was significantly up-regulated in NSCLC tissues compared to adjacent non-cancerous tissues.
  • p29 expression positively correlated with clinical stage, histological differentiation, and Ki-67 expression.
  • High p29 levels were associated with poor overall survival in NSCLC patients.
  • Interference of p29 blocked S phase entry, inhibited A549 cell proliferation, and increased p21 expression.

Conclusions:

  • p29 contributes to NSCLC progression by enhancing cell proliferation.
  • Targeting p29 may represent a potential therapeutic strategy for NSCLC.
Keywords:
NSCLCPrognosisProliferationp29/SYF2

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