Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromosome Structure02:40

Chromosome Structure

28.2K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
28.2K
Chromosome Structure02:40

Chromosome Structure

6.8K
6.8K
Chromatin Packaging02:21

Chromatin Packaging

23.7K
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...
23.7K
Chromatin Packaging01:32

Chromatin Packaging

20.5K
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
20.5K
Karyotyping01:17

Karyotyping

70.9K
Overview
70.9K
The Nucleosome01:19

The Nucleosome

4.9K
Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Specificity and exon target space of splicing modifying compounds.

Nature communications·2026
Same author

CRISPRi screening identifies SON and MAP4K1 as regulators of type III cytokine expression in innate lymphoid cells.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

Centromeric α-satellite DNA is a hotspot of genotoxic damage, incomplete repair, and cytoplasmic mislocalization.

bioRxiv : the preprint server for biology·2026
Same author

Optimization of Genome-Wide CRISPR Screens Using Dual-Guide RNA Infection with Cas9 Electroporation (DICE).

The CRISPR journal·2026
Same author

Stabilizing and strengthening the US physician-scientist faculty workforce in academic medicine: a proposed institutional framework.

JCI insight·2026
Same author

Lignocellulose-mediated selection of potential halophilic PET-degrading enzymes from mangrove soil.

Nature communications·2026

Related Experiment Video

Updated: Apr 20, 2026

Capturing Chromosome Conformation Across Length Scales
10:15

Capturing Chromosome Conformation Across Length Scales

Published on: January 20, 2023

4.3K

HCoDES reveals chromosomal DNA end structures with single-nucleotide resolution.

Yair Dorsett1, Yanjiao Zhou2, Anthony T Tubbs1

  • 1Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Molecular Cell
|December 2, 2014
PubMed
Summary

DNA double-strand break (DSB) repair pathway choice depends on DNA end structure. A new method, hairpin capture of DNA end structures (HCoDES), reveals unexpected 5' overhangs, suggesting distinct roles for H2AX and 53BP1 in DNA repair.

More Related Videos

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

4.9K
Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

21.5K

Related Experiment Videos

Last Updated: Apr 20, 2026

Capturing Chromosome Conformation Across Length Scales
10:15

Capturing Chromosome Conformation Across Length Scales

Published on: January 20, 2023

4.3K
Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

4.9K
Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

21.5K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • DNA double-strand breaks (DSBs) are critical DNA lesions.
  • The structure of broken DNA ends dictates the repair pathway.
  • Understanding DSB end structures is crucial for comprehending genome stability.

Purpose of the Study:

  • To develop a high-resolution method for elucidating chromosomal DNA end structures.
  • To define the structures of DNA ends generated by physiologic DSBs and genome editing nucleases.
  • To investigate the role of H2AX and 53BP1 in DNA end processing.

Main Methods:

  • Development of hairpin capture of DNA end structures (HCoDES).
  • Application of HCoDES to analyze DNA end structures at single-nucleotide resolution.
  • Analysis of G1 phase cells deficient in H2AX or 53BP1.

Main Results:

  • HCoDES successfully defined DNA end structures of physiologic and engineered DSBs.
  • DNA ends in H2AX or 53BP1 deficient cells showed extensive resection, forming long single-stranded overhangs.
  • Unexpectedly long 5' single-stranded overhangs were observed in addition to 3' overhangs.
  • Divergent DNA end structures suggest distinct functions for H2AX and 53BP1 in end protection.

Conclusions:

  • HCoDES provides high-resolution insights into DNA end structures during DSB repair.
  • The findings reveal complex DNA end processing pathways.
  • Distinct roles for H2AX and 53BP1 in managing DNA end structures are proposed.